This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUS252
catalog :
127674
product information
Catalog Number :
127674
Product Name :
pUS252
article :
doi
id28229534
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepK18
backbone_mutation
backbone_origin
backbone_size2620
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The creators (Nick Coleman & Mark Somerville) request that their work is cited once published. Please follow Addgene's How to Cite guidelines below.
growth strain :
Expresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbones
growth temp :
Based on what we know from other high-copy GFP-expressing vectors, there is a chance of vector instability (deletions / loss of GFP gene), although we havent seen this for this plasmid. Reducing growth temperature to 30 C can help to prevent this, since it lowers the copy number of the plasmid.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII, PstI, SphI, NheI
cloning_site_5BamHI, EcoRI, XbaI, XhoI, NdeI
promoterlac
sequencing_primer_3M13 fwd
sequencing_primer_5M13 rev
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameFree Use GFP (fuGFP)
shRNA_sequence
size739
species
100
Synthetic
tags
resistance markers :
3103
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA