This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
yeast_DualReporter
catalog :
127546
citations: 1
Reference
Vaishnav E, de Boer C, Molinet J, Yassour M, Fan L, Adiconis X, et al. The evolution, evolvability and engineering of gene regulatory DNA. Nature. 2022;603:455-463 pubmed publisher
product information
Catalog Number :
127546
Product Name :
yeast_DualReporter
article :
doi10.1101/224907
id28203492
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepPAL1_His3core
backbone_mutationyEVenus promoter replaced with minimal promoter and multiple cloning site.
backbone_originSharon et al NBT 2012 (Eran Segal's group)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Synthetic Biology
growth strain :
A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
origin :
37
pi :
alt_names
ampicillin resistance
AmpR
beta-lactamase
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterbla (E. coli)
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namebla
shRNA_sequence
size861
species
32644
Other
bacteria
tags
alt_names
RFP
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterTEF2
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namemCherry
shRNA_sequence
size711
species
100
Synthetic
tags
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterURA3
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYEL021W
geneURA3
id856692
genbank_ids
mutation
nameURA3
shRNA_sequence
size804
species
4932
Saccharomyces cerevisiae
tags
alt_names
YFP
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterpTpA+MCS
sequencing_primer_3cggagacagaaaatttgtg
sequencing_primer_5agtgttgttccagtttgg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameyEVenus
shRNA_sequence
size717
species
100
Synthetic
tags
alt_names
nourseothricin resistance
nourseothricin acetyltransferase
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterTEF
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameNat1
shRNA_sequence
size
species
32644
Other
tags
resistance markers :
3145
tags :
Unknown
terms :
URA3
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA