This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Sox2-t2A-GFP
catalog :
127537
citations: 3
Reference
Silvennoinen K, Gawel K, Tsortouktzidis D, Pitsch J, Alhusaini S, Van Loo K, et al. SCN1A overexpression, associated with a genomic region marked by a risk variant for a common epilepsy, raises seizure susceptibility. Acta Neuropathol. 2022;144:107-127 pubmed publisher
Voigt E, Wallenburg M, Wollenzien H, Thompson E, Kumar K, Feiner J, et al. Sox2 Is an Oncogenic Driver of Small-Cell Lung Cancer and Promotes the Classic Neuroendocrine Subtype. Mol Cancer Res. 2021;19:2015-2025 pubmed publisher
Dhaliwal N, Abatti L, Mitchell J. KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control. Genes Dev. 2019;: pubmed publisher
product information
Catalog Number :
127537
Product Name :
Sox2-t2A-GFP
article :
doi10.1101/gad.324319.119
id28203486
pubmed_id31221664
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3-TOPO
backbone_mutationInserted T2a-eGFP at 3' terminal of MCS.
backbone_originInvitrogen
backbone_size6150
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses human SOX2 and eGFP via t2A linker.
origin :
37
pi :
alt_names
ANOP3
MCOPS3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3cgtcgccgtccagctcgaccag
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
NM_003106
mutation
nameSOX2
shRNA_sequence
size954
species
9606
Homo sapiens
tags
plasmid copy :
SOX2 was subcloned from Harvard Institute of Proteomics (HsCD00079917)
resistance markers :
4497
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA