This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAVA-LGF2(fs)-Gal11p(fs)
catalog :
127483
citations: 1
Reference
Andrews S, Schaefer Ramadan S, Al Thani N, Ahmed I, Mohamoud Y, Malek J. High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq). J Biol Chem. 2019;: pubmed publisher
product information
Catalog Number :
127483
Product Name :
pAVA-LGF2(fs)-Gal11p(fs)
article :
doi10.1074/jbc.RA119.008792
id28203475
pubmed_id31182485
bacterial resistance :
Chloramphenicol
cloning :
backbonepBT
backbone_mutationinserted AD domain of pTRG (Agilent #240066) adjacent but convergent to the DBD
backbone_originAgilent #240067
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction
growth temp :
The Malek lab recommends using BacterioMatch II Validation Reporter Competent Cells (Catalog #200192) for downstream applications.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BstXI
cloning_site_5BstXI
promoter
sequencing_primer_3AGCTTCCAGTTGTTCAGCCA
sequencing_primer_5TCCGTTGTGGGGAAAGTTATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYOL051W, ABE1, MED15, RAR3, SDS4, SPT13
geneGAL11
id854106
genbank_ids
mutationOne nucleotide inserted between lambda CI and LGF2. One nucleotide inserted between Gal11p and RNAp.
nameLGF2 (frame shift)-Gal11p (frame shift)
shRNA_sequence
size421
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
4495
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA