This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX330-sgR26
catalog :
127376
product information
Catalog Number :
127376
Product Name :
pX330-sgR26
article :
doi10.3389/fimmu.2020.00057
id28203455
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepX330-U6-Chimeric_BB-CBh-hSpCas9
backbone_mutationInsertion of gRNA targeting intron 1 of the mouse ROSA26 locus.
backbone_originFeng Zhang laboratory
backbone_size8506
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mouse Targeting
CRISPR
growth strain :
Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3tagagccatttgtctgcagaattgg
sequencing_primer_5not available
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesGtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1
geneGt(ROSA)26Sor
id14910
genbank_ids
mutation
nameROSA26
shRNA_sequenceACTCCAGTCTTTCTAGAAGA
size
species
10090
Mus musculus
tags
plasmid copy :
The backbone vector was received from Addgene (Addgene #42230)
resistance markers :
4489
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA