This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)/AUG-DAP5-3XFLAG
catalog :
127331
citations: 1
Reference
Kearse M, Goldman D, Choi J, Nwaezeapu C, Liang D, Green K, et al. Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors. Genes Dev. 2019;33:871-885 pubmed publisher
product information
Catalog Number :
127331
Product Name :
pcDNA3.1(+)/AUG-DAP5-3XFLAG
article :
doi10.1101/gad.324715.119
id28203444
pubmed_id31171704
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: Plasmid contains a 23 nucleotide deletion in the backbone compared to the depositor sequence, which does not affect plasmid function.
growth strain :
Express 3xFLAG tagged DAP5 protein from AUG start codon
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAAG1, DAP5, NAT1, P97
geneEIF4G2
id1982
genbank_ids
mutation
nameDAP5
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tag3xFLAG
resistance markers :
2108
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA