pLVX-UbC-rtTA-Ngn2:2A:Ascl1 | Addgene 127289 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVX-UbC-rtTA-Ngn2:2A:Ascl1

catalog :

127289

citations: 5

Reference
Herdy J, Traxler L, Agarwal R, Karbacher L, Schlachetzki J, Boehnke L, et al. Increased post-mitotic senescence in aged human neurons is a pathological feature of Alzheimer's disease. Cell Stem Cell. 2022;29:1637-1652.e6 pubmed publisher
Traxler L, Herdy J, Stefanoni D, Eichhorner S, Pelucchi S, Sz xfc cs A, et al. Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease. Cell Metab. 2022;34:1248-1263.e6 pubmed publisher
B xf6 hnke L, Zhou Yang L, Pelucchi S, Kogler F, Frantal D, Sch xf6 n F, et al. Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion. Cell Reprogram. 2022;24:304-313 pubmed publisher
Mertens J, Herdy J, Traxler L, Schäfer S, Schlachetzki J, Böhnke L, et al. Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patients. Cell Stem Cell. 2021;: pubmed publisher
Herdy J, Schäfer S, Kim Y, Ansari Z, Zangwill D, Ku M, et al. Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. elife. 2019;8: pubmed publisher

product information

Catalog Number :

127289

Product Name :

pLVX-UbC-rtTA-Ngn2:2A:Ascl1

article :

doi	10.7554/eLife.41356
id	28203439
pubmed_id	31099332

bacterial resistance :

Ampicillin

cloning :

backbone

pLVX-tight-puro and pLVX-rtTA(advanced)

backbone_mutation

vector contains both the rtTA(advanced) derived from pLVX-rtTA(advanced) (Clontech) driven by the UbC promoter, as well as Ngn2:2A:Ascl1 driven by the TREtight promoter

backbone_origin

Clontech

backbone_size

9766

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

origin :

pi :

alt_names

Neurogenin 2, NEUROG2

Mash1

cloning

entrez_gene

aliases	ASH1, HASH1, MASH1, bHLHa46
gene	ASCL1
id	429

aliases	Atoh4, Math4A, NGN2, bHLHa8, ngn-2
gene	NEUROG2
id	63973

genbank_ids

NM_004316.4

NM_024019.4

mutation

name

Ngn2:2A:Ascl1

shRNA_sequence

size

1600

species

9606

Homo sapiens