This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DCT.VV.Orf35
catalog :
12723
citations: 1
Reference
Tscharke D, Karupiah G, Zhou J, Palmore T, Irvine K, Haeryfar S, et al. Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines. J Exp Med. 2005;201:95-104 pubmed
product information
Catalog Number :
12723
Product Name :
DCT.VV.Orf35
article :
doi10.1084/jem.20041912
id963
pubmed_id15623576
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 3.1D V5-His-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size5514
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
growth strain :
This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
origin :
37
pi :
alt_names
VV_WR_095
ribose 2'-Omethyltransferase
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5Universal T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesVACWR095
geneJ3R
id3707551
genbank_ids
mutation
nameVV.Orf35
shRNA_sequence
size1002
species
32644
Other
Vaccinia Virus
tags
resistance markers :
27
601
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA