This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PB-GFP-Gal8
catalog :
127191
citations: 4
Reference
Zhu Y, Ma J, Shen R, Lin J, Li S, Lu X, et al. Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity. Nat Biomed Eng. 2023;: pubmed publisher
Duan J, Bao C, Xie Y, Guo H, Liu Y, Li J, et al. Targeted core-shell nanoparticles for precise CTCF gene insert in treatment of metastatic breast cancer. Bioact Mater. 2022;11:1-14 pubmed publisher
Karlsson J, Tzeng S, Hemmati S, Luly K, Choi O, Rui Y, et al. Photocrosslinked Bioreducible Polymeric Nanoparticles for Enhanced Systemic siRNA Delivery as Cancer Therapy. Adv Funct Mater. 2021;31: pubmed publisher
Rui Y, Wilson D, Choi J, Varanasi M, Sanders K, Karlsson J, et al. Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing. Sci Adv. 2019;5:eaay3255 pubmed publisher
product information
Catalog Number :
127191
Product Name :
PB-GFP-Gal8
article :
doi10.1126/sciadv.aay3255
id28210808
pubmed_id31840076
bacterial resistance :
Ampicillin
cloning :
backbonePB-CAG-GAPd2
backbone_mutationIn-frame insertion of Gal8 to form c-terminus fusion protein with GFP
backbone_origin
backbone_size6466
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
origin :
37
pi :
alt_names
Galectin-8
GFP
LGALS8
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BsrGI
promoterCMV
sequencing_primer_3AAGATCTCAGTGGTATTTGTGAG
sequencing_primer_5CATGGTCCTGCTGGAGTTCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGal-8, PCTA-1, PCTA1, Po66-CBP
geneLGALS8
id3964
genbank_ids
3964
mutation
nameGFP-Gal8
shRNA_sequence
size2135
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagGal8 is fused to the c-terminus of GFP
resistance markers :
4068
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA