(SUMO)5-(SIM)10 | Addgene 126923 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

(SUMO)5-(SIM)10

catalog :

126923

citations: 1

Reference
Banani S, Rice A, Peeples W, Lin Y, Jain S, Parker R, et al. Compositional Control of Phase-Separated Cellular Bodies. Cell. 2016;166:651-663 pubmed publisher

product information

Catalog Number :

126923

Product Name :

(SUMO)5-(SIM)10

article :

doi	10.1016/j.cell.2016.06.010
id	28196724
pubmed_id	27374333

bacterial resistance :

Ampicillin

cloning :

backbone

pMal-tev

backbone_mutation

tev sites added on either side of the NdeI / BamHI cloning site C-terminal His6 added, HindIII site added after C-terminal His6

backbone_origin

NEB

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHi and BglII
cloning_site_5	NdeI
promoter	tac
sequencing_primer_3	GTAAAACGACGGCCAGT
sequencing_primer_5	TGCGTACTGCGGTGATCAAC'
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	SMT3A, SMT3H1, SUMO-3, Smt3B
gene	SUMO3
id	6612

genbank_ids

mutation

internal BamHI site between (SUMO)5 and (SIM)10

name

5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4

shRNA_sequence

size

2625

species

9606

Homo sapiens