This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRSFDuet1-Cdc45 (delta 154 164)
catalog :
126879
citations: 1
Reference
Simon A, Sannino V, Costanzo V, Pellegrini L. Structure of human Cdc45 and implications for CMG helicase function. Nat Commun. 2016;7:11638 pubmed publisher
product information
Catalog Number :
126879
Product Name :
pRSFDuet1-Cdc45 (delta 154 164)
article :
doi10.1038/ncomms11638
id28203372
pubmed_id27189187
bacterial resistance :
Kanamycin
cloning :
backbonepRSFDuet-1
backbone_mutation
backbone_origin
backbone_size3766
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses crystallisation construct of human Cdc45 in bacteria
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7 promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TTGTACACGGCCGCATAATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDC45L, CDC45L2, MGORS7, PORC-PI-1
geneCDC45
id8318
genbank_ids
mutationdeletion amino acid 154-164
nameHuman Cdc45
shRNA_sequence
size1677
species
9606
Homo sapiens
tags
location
tagNo tag
plasmid copy :
This clone was already present before I joined the lab. But I have fully sequenced it to confirm.
resistance markers :
3794
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA