This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
catalog :
126706
citations: 1
Reference
Kwong G, Marquez H, Yang C, Wong J, Kotton D. Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering. Stem Cell Reports. 2019;13:499-514 pubmed publisher
product information
Catalog Number :
126706
Product Name :
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
article :
doi10.1016/j.stemcr.2019.07.014
id28212036
pubmed_id31422908
bacterial resistance :
Ampicillin
cloning :
backbonePX458
backbone_mutation
backbone_originMorrissey Laboratory
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence
origin :
37
pi :
alt_names
ACTA2
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACTSA
geneACTA2
id59
genbank_ids
mutation
nameACTA2_gRNA1-SpCas9-T2A-GF
shRNA_sequenceunknown
size
species
9606
Homo sapiens
tags
resistance markers :
706
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA