This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pB-bsd-Flag-Trl1
catalog :
126609
citations: 1
Reference
Unlu I, Lu Y, Wang X. The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress. J Biol Chem. 2018;293:19365-19376 pubmed publisher
product information
Catalog Number :
126609
Product Name :
pB-bsd-Flag-Trl1
article :
doi10.1074/jbc.RA118.004872
id28203357
pubmed_id30355738
bacterial resistance :
Ampicillin
cloning :
backbonePB-bsd
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Construct contains a non-canonical FLAG tag of DYKDDDDR rather than the typical DYKDDDDK that is functional in the experience of the depositor.
growth strain :
Expresses N-terminal Flag-tagged yeast Trl1
origin :
37
pi :
alt_names
Trl1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterCAG
sequencing_primer_3
sequencing_primer_5GAGCCTCTGCTAACCATGTTCATGCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYJL087C, LIG1, RLG1
geneTRL1
id853358
genbank_ids
mutation
nametRNA ligase 1
shRNA_sequence
size2484
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagFlag
resistance markers :
4487
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA