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product summary
company name :
Addgene
product type :
cDNA
product name :
pDmelOR-mRho.V5.mER.hOr56a
catalog :
126479
citations: 1
Reference
Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson B, et al. Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells. Chem Senses. 2019;44:673-682 pubmed publisher
product information
Catalog Number :
126479
Product Name :
pDmelOR-mRho.V5.mER.hOr56a
article :
doi10.1093/chemse/bjz062
id28203333
pubmed_id31504297
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-BI
backbone_mutation
backbone_origin
backbone_size4111
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Is it recommended to digest the vector with XhoI and NaeI and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint
growth strain :
Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells
origin :
37
pi :
alt_names
DmelOr56a
Odorant receptor 56a
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3GGCCTATATAAGCAGAGCTCGTTT
sequencing_primer_5CATTTTATGTTTCAGGTTCAGGGGG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDmel_CG12501, 56E.1, 56a, CG12501, DOR59, DmOr56a, Dmel CG12501, OR56a
geneOr56a
id37269
genbank_ids
mutationcodon optimization for H. sapiens
nameOr56a
shRNA_sequence
size1338
species
7227
Drosophila melanogaster
tags
locationN terminal on insert
tagH. sapiens Rhodopsin 344QVAPA348 (mRho)
locationN terminal on insert
tagV5 tag
locationN terminal on insert
tagH. sapiens HCN1 106VNKFSL111 (mER)
alt_names
DmelOrco
Olfactory receptor co-receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoterCMV
sequencing_primer_3GTGGTATGGCTGATTATGATCCTCT
sequencing_primer_5GTGTACGGTGGGAGGTCTATATAAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG10609, 83A.2, 83b, A45, CG10609, DOR45, DOR83b, DmORCO, DmOrco, DmelOrco, Dmel CG10609, OR49, OR83B, OR83b, ORCO, Or83b, OrCo, or83b, orco, vainsA
geneOrco
id40650
genbank_ids
mutationcodon optimization for H. sapiens; including a recombinant -globin/IgG chimeric intron
nameOrco
shRNA_sequence
size1509
species
7227
Drosophila melanogaster
tags
locationN terminal on insert
tagMyc
resistance markers :
4488
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA