pDmelOR | Addgene 126476 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDmelOR

catalog :

126476

citations: 1

Reference
Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson B, et al. Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells. Chem Senses. 2019;44:673-682 pubmed publisher

product information

Catalog Number :

126476

Product Name :

pDmelOR

article :

doi	10.1093/chemse/bjz062
id	28203333
pubmed_id	31504297

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-BI

backbone_mutation

backbone_origin

backbone_size

4111

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Is it recommended to digest the vector with XhoI and PvuI-HF and gel purify the two bands before sequencing as the two CMV promoters and SV40-polyA sequences are very similar to each other. Use the EcoRI restriction site for best functional expression on the MCS2. Please visit https://www.biorxiv.org/content/10.1101/669127v1 for BioRxiv preprint

growth strain :

Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a -globin/IgG chimeric intron

origin :

pi :

alt_names

Orco

Odorant receptor co-receptor

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	GTGGTATGGCTGATTATGATCCTCT
sequencing_primer_5	GTGTACGGTGGGAGGTCTATATAAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG10609, 83A.2, 83b, A45, CG10609, DOR45, DOR83b, DmORCO, DmOrco, DmelOrco, Dmel CG10609, OR49, OR83B, OR83b, ORCO, Or83b, OrCo, or83b, orco, vainsA
gene	Orco
id	40650

genbank_ids

mutation

codon optimization for H. sapiens; includes a recombinant -globin/IgG chimeric intron to inhibit its functional expression in bacteria

name

DmelOrco

shRNA_sequence

size

1509

species

7227

Drosophila melanogaster