This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
UbcH5c WT pET28a
catalog :
12643
citations: 8
Reference
Umano A, Fang K, Qu Z, Scaglione J, Altınok S, Treadway C, et al. The molecular basis of spinocerebellar ataxia type 48 caused by a de novo mutation in the ubiquitin ligase CHIP. J Biol Chem. 2022;298:101899 pubmed publisher
Kanack A, Olp M, Newsom O, Scaglione J, Gooden D, McMahon K, et al. Chemical Regulation of the Protein Quality Control E3 Ubiquitin Ligase C-Terminus of Hsc70 Interacting Protein (CHIP). Chembiochem. 2022;23:e202100633 pubmed publisher
Kudriaeva A, Saratov G, Kaminskaya A, Vladimirov V, Barzilovich P, Belogurov A. Polyamines Counteract Carbonate-Driven Proteasome Stalling in Alkaline Conditions. Biomolecules. 2020;10: pubmed publisher
Kanack A, Vittal V, Haver H, Keppel T, Gundry R, Klevit R, et al. UbcH5 Interacts with Substrates to Participate in Lysine Selection with the E3 Ubiquitin Ligase CHIP. Biochemistry. 2020;59:2078-2088 pubmed publisher
Kanack A, Newsom O, SCAGLIONE K. Most mutations that cause spinocerebellar ataxia autosomal recessive type 16 (SCAR16) destabilize the protein quality-control E3 ligase CHIP. J Biol Chem. 2018;293:2735-2743 pubmed publisher
Atipairin A, Ratanaphan A. In Vitro Enhanced Sensitivity to Cisplatin in D67Y BRCA1 RING Domain Protein. Breast Cancer (Auckl). 2011;5:201-8 pubmed publisher
Atipairin A, Canyuk B, Ratanaphan A. Substitution of aspartic acid with glutamic acid at position 67 of the BRCA1 RING domain retains ubiquitin ligase activity and zinc(II) binding with a reduced transition temperature. J Biol Inorg Chem. 2011;16:217-26 pubmed publisher
Brzovic P, Lissounov A, Christensen D, Hoyt D, Klevit R. A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Mol Cell. 2006;21:873-80 pubmed
product information
Catalog Number :
12643
Product Name :
UbcH5c WT pET28a
article :
doi10.1016/j.molcel.2006.02.008
id945
pubmed_id16543155
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originstratagene
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
UbcH5c
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoter
sequencing_primer_3
sequencing_primer_5universal T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesE2(17)KB3, UBC4/5, UBCH5C
geneUBE2D3
id7323
genbank_ids
mutation
nameUbcH5c - Ubiquitin conjugating enzyme
shRNA_sequence
size444
species
9606
Homo sapiens
tags
resistance markers :
261
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA