This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LPCX Smad3 deltaSSVS
catalog :
12639
citations: 2
Reference
Zhou J, Dabiri Y, Gama Brambila R, Ghafoory S, Altinbay M, Mehrabi A, et al. pVHL-mediated SMAD3 degradation suppresses TGF-β signaling. J Cell Biol. 2022;221: pubmed publisher
Choy L, Skillington J, Derynck R. Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. J Cell Biol. 2000;149:667-82 pubmed
product information
Catalog Number :
12639
Product Name :
LPCX Smad3 deltaSSVS
article :
doi10.1083/jcb.149.3.667
id943
pubmed_id10791980
bacterial resistance :
Ampicillin
cloning :
backboneLPCX
backbone_mutation
backbone_origin
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
LPCX was made by replacing the neo gene of LNCX with the puro gene.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HpaI
cloning_site_5HpaI
promoter
sequencing_primer_3
sequencing_primer_5LNCX
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
geneSMAD3
id4088
genbank_ids
mutationLast 4 aa's deleted.
nameSmad3
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
115
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA