This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK DPC4 Flag (aka: Smad4 Flag)
catalog :
12627
citations: 3
Reference
Principe D, Underwood P, Kumar S, Timbers K, Koch R, Trevino J, et al. Loss of SMAD4 Is Associated With Poor Tumor Immunogenicity and Reduced PD-L1 Expression in Pancreatic Cancer. Front Oncol. 2022;12:806963 pubmed publisher
Samba Mondonga M, Calvé A, Mallette F, Santos M. MyD88 Regulates the Expression of SMAD4 and the Iron Regulatory Hormone Hepcidin. Front Cell Dev Biol. 2018;6:105 pubmed publisher
Zhang Y, Feng X, We R, Derynck R. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Nature. 1996;383:168-72 pubmed
product information
Catalog Number :
12627
Product Name :
pRK DPC4 Flag (aka: Smad4 Flag)
article :
doi10.1038/383168a0
id942
pubmed_id8774881
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
PCR'd human placental library to get 5' end of DPC4 sequences using primers, then combined with an insert of Image clone 23275 to get full-length DPC4. See author's map for details.
origin :
37
pi :
alt_names
Smad4
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDPC4, JIP, MADH4, MYHRS
geneSMAD4
id4089
genbank_ids
mutation
nameDPC4
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagflag
resistance markers :
115
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA