This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-AR-R405A
catalog :
126052
citations: 1
Reference
Lagarde W, Blackwelder A, Minges J, Hnat A, French F, Wilson E. Androgen receptor exon 1 mutation causes androgen insensitivity by creating phosphorylation site and inhibiting melanoma antigen-A11 activation of NH2- and carboxyl-terminal interaction-dependent transactivation. J Biol Chem. 2012;287:10905-15 pubmed publisher
product information
Catalog Number :
126052
Product Name :
pCMV-AR-R405A
article :
doi10.1074/jbc.M111.336081
id28203314
pubmed_id22334658
bacterial resistance :
Ampicillin
cloning :
backbonepCMV5
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The human AR N-terminal glutamine repeat may undergo minor expansion or contraction during bacterial propagation. It may be advisable to limit the time of bacterial growth and to verify glutamine repeat length using Sanger sequencing. Minor changes in AR N-terminal glutamine repeat length should not significantly alter AR activity.
growth strain :
Expresses human androgen receptor (R405A) in mammalian cells
origin :
37
pi :
alt_names
androgen receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
geneAR
id367
genbank_ids
mutationR405A
nameAR
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
3305
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA