This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV mSin1.2 HA
catalog :
12583
citations: 2
Reference
Pergolizzi B, Panuzzo C, Ali M, Lo Iacono M, Levra Levron C, Ponzone L, et al. Two conserved glycine residues in mammalian and Dictyostelium Rictor are required for mTORC2 activity and integrity. J Cell Sci. 2019;132: pubmed publisher
Frias M, Thoreen C, Jaffe J, Schroder W, Sculley T, Carr S, et al. mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Curr Biol. 2006;16:1865-70 pubmed
product information
Catalog Number :
12583
Product Name :
pMSCV mSin1.2 HA
article :
doi10.1016/j.cub.2006.08.001
id925
pubmed_id16919458
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV
backbone_mutation
backbone_originClontech
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Human mSin1 isoforms were cloned by RT-PCR from mRNA isolated from HEK-293T cells. This plasmid encodes isoform 2. See author's map for more information.
origin :
37
pi :
alt_names
mSin1 transcript variant protein isoform 2
MAPKAP1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI/SalI
promoter
sequencing_primer_3
sequencing_primer_5MSCV primer
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesJC310, MIP1, SIN1, SIN1b, SIN1g
geneMAPKAP1
id79109
genbank_ids
NP_077022
mutation
namemSin1.2
shRNA_sequence
size1527
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
resistance markers :
432
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA