pMSCV mSin1.1 HA | Addgene 12582 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMSCV mSin1.1 HA

catalog :

12582

citations: 3

Reference
Kovalski J, Bhaduri A, Zehnder A, Neela P, Che Y, Wozniak G, et al. The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Mol Cell. 2019;73:830-844.e12 pubmed publisher
Glidden E, Gray L, Vemuru S, Li D, Harris T, Mayo M. Multiple site acetylation of Rictor stimulates mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of Akt protein. J Biol Chem. 2012;287:581-8 pubmed publisher
Frias M, Thoreen C, Jaffe J, Schroder W, Sculley T, Carr S, et al. mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Curr Biol. 2006;16:1865-70 pubmed

product information

Catalog Number :

12582

Product Name :

pMSCV mSin1.1 HA

article :

doi	10.1016/j.cub.2006.08.001
id	925
pubmed_id	16919458

bacterial resistance :

Ampicillin

cloning :

backbone

pMSCV

backbone_mutation

backbone_origin

Clontech

backbone_size

6300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Human mSin1 isoforms were cloned by RT-PCR from mRNA isolated from HEK-293T cells. This plasmid encodes isoform 1. See author's map for more information.

origin :

pi :

alt_names

mSin1 transcript variant protein isoform 1

MAPKAP1

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	XhoI/SalI
promoter
sequencing_primer_3
sequencing_primer_5	MSCV primer
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	JC310, MIP1, SIN1, SIN1b, SIN1g
gene	MAPKAP1
id	79109

genbank_ids

NP_001006618

mutation

name

mSin1.1

shRNA_sequence

size

1633

species

9606

Homo sapiens