This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV mSin1.2 myc
catalog :
12577
citations: 2
Reference
Glidden E, Gray L, Vemuru S, Li D, Harris T, Mayo M. Multiple site acetylation of Rictor stimulates mammalian target of rapamycin complex 2 (mTORC2)-dependent phosphorylation of Akt protein. J Biol Chem. 2012;287:581-8 pubmed publisher
Frias M, Thoreen C, Jaffe J, Schroder W, Sculley T, Carr S, et al. mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Curr Biol. 2006;16:1865-70 pubmed
product information
Catalog Number :
12577
Product Name :
pMSCV mSin1.2 myc
article :
doi10.1016/j.cub.2006.08.001
id925
pubmed_id16919458
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV
backbone_mutation
backbone_originClontech
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Human mSin1 isoforms were cloned by RT-PCR from mRNA isolated from HEK-293T cells. This plasmid encodes isoform 2. See author's map for more information.
origin :
37
pi :
alt_names
mSin1 transcript variant protein isoform 2
MAPKAP1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI/SalI
promoter
sequencing_primer_3
sequencing_primer_5MSCV primer
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesJC310, MIP1, SIN1, SIN1b, SIN1g
geneMAPKAP1
id79109
genbank_ids
NP_077022
mutation
namemSin1.2
shRNA_sequence
size1537
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmyc
resistance markers :
432
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA