This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cytosolic Syk Biosensor
catalog :
125729
citations: 2
Reference
Yang J, Chi W, Liang J, Takayanagi S, Iglesias P, Huang C. Deciphering cell signaling networks with massively multiplexed biosensor barcoding. Cell. 2021;184:6193-6206.e14 pubmed publisher
Xiang X, Sun J, Wu J, He H, Wang Y, Zhu C. A FRET-Based Biosensor for Imaging SYK Activities in Living Cells. Cell Mol Bioeng. 2011;4:670-677 pubmed
product information
Catalog Number :
125729
Product Name :
Cytosolic Syk Biosensor
article :
doi10.1007/s12195-011-0211-x
id28203279
pubmed_id25541586
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutationThe original fragment between HindIII and EcoRI in pcDNA3.1 is replaced by the following fragment GCGGCCGCCACCATG GTG CGG GGT TCT CATCAT CAT CATCATCATGGTATGGCT AGC ATG ACT GGT GGA CAG CAA ATG GGT CCG GAT CTG TAC GAC GAT AAG GAT CCC AGC TCG AGA TCT GCA GCT GGT ACC ATG containing a Kozak sequence and 6xHistine tag.
backbone_originInvitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego
backbone_size5505
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
FRET biosensor for studying SYK activities in cells
origin :
37
pi :
alt_names
ZAP70 biosensor
cloning
entrez_gene
aliasesADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70
geneZAP70
id7535
genbank_ids
mutation
nameSYK biosensor
shRNA_sequence
size1782
species
100
Synthetic
tags
location
tag6xHistine Tag at the N-terminal.
resistance markers :
2854
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA