This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p2XStrep-ATIC
catalog :
125685
citations: 1
Reference
Liu C, Knudsen G, Pedley A, He J, Johnson J, Yaron T, et al. Mapping Post-Translational Modifications of de Novo Purine Biosynthetic Enzymes: Implications for Pathway Regulation. J Proteome Res. 2019;18:2078-2087 pubmed publisher
product information
Catalog Number :
125685
Product Name :
p2XStrep-ATIC
article :
doi10.1021/acs.jproteome.8b00969
id28197765
pubmed_id30964683
bacterial resistance :
Ampicillin
cloning :
backbonepCI-neo
backbone_mutationaddition of 2X Strep-tag II by restriction cloning at the beginning of the MCS
backbone_originClontech
backbone_size5472
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses human ATIC protein with an N-terminal 2XStrep affinity tag
origin :
37
pi :
alt_names
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5GAC ATC CAC TTT GCC TTT CT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH
geneATIC
id471
genbank_ids
NM_004044
mutation
nameATIC
shRNA_sequence
size1779
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag2X Strep-Tag II
resistance markers :
3618
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA