This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PCD FL1P
catalog :
12567
citations: 1
Reference
Wang B, Love T, Call M, Doench J, Novina C. Recapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006;22:553-60 pubmed
product information
Catalog Number :
12567
Product Name :
PCD FL1P
article :
doi10.1016/j.molcel.2006.03.034
id923
pubmed_id16713585
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size7200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
Other
Reporter for translation repression
growth notes :
Firefly luciferase was cloned into the HindIII/SpeI sites of pcDNA3.1. One perfect complementary binding site to the antisense strand of CXCR4 siRNA was cloned in the XbaI/ApaI sites in the 3'UTR of luciferase. Perfect binding site sequence is - 5'-AAGTTTTCACTCCAGCTAACA
origin :
37
pi :
alt_names
CXCR4
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5XbaI
promoter
sequencing_primer_3BGH rev primer
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1
geneCXCR4
id7852
genbank_ids
mutation
nameOne perfect CXCR4 binding site
shRNA_sequence
size40
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagluciferase
resistance markers :
366
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA