PCD FL6X | Addgene 12566 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PCD FL6X

catalog :

12566

citations: 3

Reference
Yadava S, Feng A, Parobchak N, Wang B, Rosen T. miR-15b-5p promotes expression of proinflammatory cytokines in human placenta by inhibiting Apelin signaling pathway. Placenta. 2021;104:8-15 pubmed publisher
Wang B, Cruz Ithier M, Parobchak N, Yadava S, Schulkin J, Rosen T. Vitamin D stimulates multiple microRNAs to inhibit CRH and other pro-labor genes in human placenta. Endocr Connect. 2018;: pubmed publisher
Wang B, Love T, Call M, Doench J, Novina C. Recapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006;22:553-60 pubmed

product information

Catalog Number :

12566

Product Name :

PCD FL6X

article :

doi	10.1016/j.molcel.2006.03.034
id	923
pubmed_id	16713585

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

7200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

Other

Reporter for translation repression

growth notes :

Firefly luciferase was cloned into the HindIII/SpeI sites of pcDNA3.1. Six imperfect complementary binding sites to the antisense strand of CXCR4 siRNA were cloned in the XbaI/ApaI sites in the 3'UTR of luciferase. Author's sequence data was obtained by using a primer reading forward from inside the luciferase gene.

origin :

pi :

alt_names

CXCR4

cloning

clone_method	Restriction Enzyme
cloning_site_3	ApaI
cloning_site_5	XbaI
promoter
sequencing_primer_3	BGH rev primer
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1
gene	CXCR4
id	7852

genbank_ids

mutation

name

Four CXCR4 imperfect binding sites

shRNA_sequence

size

140

species

9606

Homo sapiens