pcDNA 3.1(-) rat C/EBP beta (LAP) | Addgene 12558 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA 3.1(-) rat C/EBP beta (LAP)

catalog :

12558

citations: 2

Reference
Masuda M, Miyazaki Anzai S, Keenan A, Shiozaki Y, Okamura K, Chick W, et al. Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells. JCI Insight. 2016;1:e88646 pubmed publisher
Hiyama A, Hiraishi S, Sakai D, Mochida J. CCAAT/enhancer binding protein Î² regulates the expression of tumor necrosis factor-Î± in the nucleus pulposus cells. J Orthop Res. 2016;34:865-75 pubmed publisher

product information

Catalog Number :

12558

Product Name :

pcDNA 3.1(-) rat C/EBP beta (LAP)

article :

doi
id	922
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1(-)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5427

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus. For more information on the C/EBPbeta isoforms, please see the original reference: Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).

origin :

pi :

alt_names

C/EBP beta

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	EcoRI
promoter
sequencing_primer_3	BGH
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Il6dbp, NF-IL6, TCF5
gene	Cebpb
id	24253

genbank_ids

mutation

name

CCAAT/Enhancer-binding Protein beta

shRNA_sequence

size

853

species

10116

Rattus norvegicus