This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe K-Ras 12V
catalog :
12544
citations: 16
Reference
Tomoshige K, Stuart W, Fink Baldauf I, Ito M, Tsuchiya T, Nagayasu T, et al. FOXA2 Cooperates with Mutant KRAS to Drive Invasive Mucinous Adenocarcinoma of the Lung. Cancer Res. 2023;83:1443-1458 pubmed publisher
Liu H, Gong Z, Li K, Zhang Q, Xu Z, Xu Y. SRPK1/2 and PP1α exert opposite functions by modulating SRSF1-guided MKNK2 alternative splicing in colon adenocarcinoma. J Exp Clin Cancer Res. 2021;40:75 pubmed publisher
Pelullo M, Nardozza F, Zema S, Quaranta R, Nicoletti C, Besharat Z, et al. Kras/ADAM17-Dependent Jag1-ICD Reverse Signaling Sustains Colorectal Cancer Progression and Chemoresistance. Cancer Res. 2019;79:5575-5586 pubmed publisher
Li S, Liu S, Deng J, Akbay E, Hai J, Ambrogio C, et al. Assessing Therapeutic Efficacy of MEK Inhibition in a KRASG12C-Driven Mouse Model of Lung Cancer. Clin Cancer Res. 2018;24:4854-4864 pubmed publisher
Ganier O, Schnerch D, Oertle P, Lim R, Plodinec M, Nigg E. Structural centrosome aberrations promote non-cell-autonomous invasiveness. EMBO J. 2018;37: pubmed publisher
Zhu S, Shanbhag V, Wang Y, Lee J, PETRIS M. A Role for The ATP7A Copper Transporter in Tumorigenesis and Cisplatin Resistance. J Cancer. 2017;8:1952-1958 pubmed publisher
Valverde A, Peñarando J, Cañas A, Lopez Sanchez L, Conde F, Guil Luna S, et al. The addition of celecoxib improves the antitumor effect of cetuximab in colorectal cancer: role of EGFR-RAS-FOXM1-?- catenin signaling axis. Oncotarget. 2017;8:21754-21769 pubmed publisher
DeSmet M, Fleet J. Constitutively active RAS signaling reduces 1,25 dihydroxyvitamin D-mediated gene transcription in intestinal epithelial cells by reducing vitamin D receptor expression. J Steroid Biochem Mol Biol. 2017;173:194-201 pubmed publisher
Valverde A, Peñarando J, Cañas A, López Sánchez L, Conde F, Hernández V, et al. Simultaneous inhibition of EGFR/VEGFR and cyclooxygenase-2 targets stemness-related pathways in colorectal cancer cells. PLoS ONE. 2015;10:e0131363 pubmed publisher
Unni A, Lockwood W, Zejnullahu K, Lee Lin S, Varmus H. Evidence that synthetic lethality underlies the mutual exclusivity of oncogenic KRAS and EGFR mutations in lung adenocarcinoma. elife. 2015;4:e06907 pubmed publisher
Garant K, Shmulevitz M, Pan L, Daigle R, Ahn D, Gujar S, et al. Oncolytic reovirus induces intracellular redistribution of Ras to promote apoptosis and progeny virus release. Oncogene. 2016;35:771-82 pubmed publisher
Leiser D, Medová M, Mikami K, Nisa L, Stroka D, Blaukat A, et al. KRAS and HRAS mutations confer resistance to MET targeting in preclinical models of MET-expressing tumor cells. Mol Oncol. 2015;9:1434-46 pubmed publisher
Tong J, Lung R, Sin F, Law P, Kang W, Chan A, et al. Characterization of rare transforming KRAS mutations in sporadic colorectal cancer. Cancer Biol Ther. 2014;15:768-76 pubmed publisher
Hu Y, Zheng M, Gali R, Tian Z, Topal Görgün G, Munshi N, et al. A novel rapid-onset high-penetrance plasmacytoma mouse model driven by deregulation of cMYC cooperating with KRAS12V in BALB/c mice. Blood Cancer J. 2013;3:e156 pubmed publisher
Mao X, Hütt Cabezas M, Orr B, Weingart M, Taylor I, Rajan A, et al. LIN28A facilitates the transformation of human neural stem cells and promotes glioblastoma tumorigenesis through a pro-invasive genetic program. Oncotarget. 2013;4:1050-64 pubmed
Khosravi Far R, White M, Westwick J, Solski P, Chrzanowska Wodnicka M, Van Aelst L, et al. Oncogenic Ras activation of Raf/mitogen-activated protein kinase-independent pathways is sufficient to cause tumorigenic transformation. Mol Cell Biol. 1996;16:3923-33 pubmed
product information
Catalog Number :
12544
Product Name :
pBabe K-Ras 12V
article :
doi
id857
pubmed_id8668210
bacterial resistance :
Ampicillin
cloning :
backbonepBabe-Puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Constructed by Aylin Ulku at the Der lab. Insert: also a 3' BamHI site immediately adjacent to the 3' SalI site.
origin :
37
pi :
alt_names
KRas
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBabe 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliases'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
geneKRAS
id3845
genbank_ids
mutation12V constituitively activated
nameK-Ras 12V
shRNA_sequence
size600
species
9606
Homo sapiens
tags
resistance markers :
114
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA