pBabe N-Ras 61K | Addgene 12543 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBabe N-Ras 61K

catalog :

12543

citations: 16

Reference
Schaufler D, Ast D, Tumbrink H, Abedpour N, Maas L, Schwäbe A, et al. Clonal dynamics of BRAF-driven drug resistance in EGFR-mutant lung cancer. NPJ Precis Oncol. 2021;5:102 pubmed publisher
Banik I, Cheng P, Dooley C, Travnickova J, Merteroglu M, Dummer R, et al. NRASQ61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells. Pigment Cell Melanoma Res. 2020;: pubmed publisher
Ogino A, Choi J, Lin M, Wilkens M, Calles A, Xu M, et al. Genomic and pathological heterogeneity in clinically diagnosed small cell lung cancer in never/light smokers identifies therapeutically targetable alterations. Mol Oncol. 2020;: pubmed publisher
Kenyon A, Gavriouchkina D, Zorman J, Chong Morrison V, Napolitani G, Cerundolo V, et al. Generation of a double binary transgenic zebrafish model to study myeloid gene regulation in response to oncogene activation in melanocytes. Dis Model Mech. 2018;11: pubmed publisher
Hosono Y, Niknafs Y, Prensner J, Iyer M, Dhanasekaran S, Mehra R, et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 2017;171:1559-1572.e20 pubmed publisher
Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
Nelson Taylor S, Le A, Yoo M, Schubert L, Mishall K, Doak A, et al. Resistance to RET-Inhibition in RET-Rearranged NSCLC Is Mediated By Reactivation of RAS/MAPK Signaling. Mol Cancer Ther. 2017;16:1623-1633 pubmed publisher
Song Z, Liu F, Zhang J. Targeting NRASQ61K mutant delays tumor growth and angiogenesis in non-small cell lung cancer. Am J Cancer Res. 2017;7:831-844 pubmed
Giannou A, Marazioti A, Kanellakis N, Giopanou I, Lilis I, Zazara D, et al. NRAS destines tumor cells to the lungs. EMBO Mol Med. 2017;9:672-686 pubmed publisher
Mulcahy Levy J, Zahedi S, Griesinger A, Morin A, Davies K, Aisner D, et al. Autophagy inhibition overcomes multiple mechanisms of resistance to BRAF inhibition in brain tumors. elife. 2017;6: pubmed publisher
Brea E, Oh C, Manchado E, Budhu S, Gejman R, Mo G, et al. Kinase Regulation of Human MHC Class I Molecule Expression on Cancer Cells. Cancer Immunol Res. 2016;4:936-947 pubmed
Danis E, Yamauchi T, Echanique K, Zhang X, Haladyna J, Riedel S, et al. Ezh2 Controls an Early Hematopoietic Program and Growth and Survival Signaling in Early T Cell Precursor Acute Lymphoblastic Leukemia. Cell Rep. 2016;14:1953-65 pubmed publisher
Garant K, Shmulevitz M, Pan L, Daigle R, Ahn D, Gujar S, et al. Oncolytic reovirus induces intracellular redistribution of Ras to promote apoptosis and progeny virus release. Oncogene. 2016;35:771-82 pubmed publisher
Catanzaro J, Sheshadri N, Pan J, Sun Y, Shi C, Li J, et al. Oncogenic Ras induces inflammatory cytokine production by upregulating the squamous cell carcinoma antigens SerpinB3/B4. Nat Commun. 2014;5:3729 pubmed publisher
Nikolos F, Thomas C, Rajapaksa G, Bado I, Gustafsson J. ER? regulates NSCLC phenotypes by controlling oncogenic RAS signaling. Mol Cancer Res. 2014;12:843-54 pubmed publisher
Khosravi Far R, White M, Westwick J, Solski P, Chrzanowska Wodnicka M, Van Aelst L, et al. Oncogenic Ras activation of Raf/mitogen-activated protein kinase-independent pathways is sufficient to cause tumorigenic transformation. Mol Cell Biol. 1996;16:3923-33 pubmed

product information

Catalog Number :

12543

Product Name :

pBabe N-Ras 61K

article :

doi
id	857
pubmed_id	8668210

bacterial resistance :

Ampicillin

cloning :

backbone

pBabe-Puro

backbone_mutation

backbone_origin

backbone_size

5169

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

The original N-Ras was cloned as a full-length cDNA (containing 5' and 3' UTRs) Bam-Bam into pCGN, and was therefore transfered via Bam cuts into other vectors, including pBABE, and orientation checked. So, a 1.2 kb band is expected. There should be about 100 bp of 5'UTR, 527 bp N-Ras, and the rest 3'UTR

origin :

pi :

alt_names

NRAS

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBABE-3
sequencing_primer_5	pBabe 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6
gene	NRAS
id	4893

genbank_ids

mutation

61K constitutively activated.

name

NRas 61K

shRNA_sequence

size

570

species

9606

Homo sapiens