pBabe puro HA PIK3CB | Addgene 12526 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBabe puro HA PIK3CB

catalog :

12526

citations: 1

Reference
Zhao J, Liu Z, Wang L, Shin E, Loda M, Roberts T. The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005;102:18443-8 pubmed

product information

Catalog Number :

12526

Product Name :

pBabe puro HA PIK3CB

article :

doi	10.1073/pnas.0508988102
id	548
pubmed_id	16339315

bacterial resistance :

Ampicillin

cloning :

backbone

pBabe puro

backbone_mutation

backbone_origin

Available at Addgene (plasmid #1764)

backbone_size

5150

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

HMS clone ID number: HsCD00025922. To construct a hemagglutinin (HA)-tagged allele of p110 beta, a cDNA clone of human PIK3CB (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.

origin :

pi :

alt_names

phosphoinositide 3-kinase

PI3K

p110 beta

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	P110BETA, PI3K, PI3KBETA, PIK3C1
gene	PIK3CB
id	5291

genbank_ids

mutation

name

PIK3CB

shRNA_sequence

size

3240

species

9606

Homo sapiens