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product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe puro HA PIK3CA E545K
catalog :
12525
citations: 18
Reference
Wu Q, Ellis H, Siravegna G, Michel A, Norden B, Fece de la Cruz F, et al. Landscape of Clinical Resistance Mechanisms to FGFR Inhibitors in FGFR2-Altered Cholangiocarcinoma. Clin Cancer Res. 2024;30:198-208 pubmed publisher
Wang M, Liu J, Liao X, Yi Y, Xue Y, Yang L, et al. The SGK3-Catalase antioxidant signaling axis drives cervical cancer growth and therapy resistance. Redox Biol. 2023;67:102931 pubmed publisher
Rios Garcia M, Meissburger B, Chan J, de Guia R, Mattijssen F, Roessler S, et al. Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2-Dependent Mitotic Cell Death. Adv Sci (Weinh). 2022;9:e2104291 pubmed publisher
Moon H, Park H, Chae M, Choi H, Kim D, Ro S. Activated TAZ induces liver cancer in collaboration with EGFR/HER2 signaling pathways. BMC Cancer. 2022;22:423 pubmed publisher
Prieto Garcia C, Hartmann O, Reissland M, Braun F, Bozkurt S, Pahor N, et al. USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Mol Oncol. 2022;: pubmed publisher
Jayashankar V, Edinger A. Macropinocytosis confers resistance to therapies targeting cancer anabolism. Nat Commun. 2020;11:1121 pubmed publisher
Kinoh H, Shibasaki H, Liu X, Yamasoba T, Cabral H, Kataoka K. Nanomedicines blocking adaptive signals in cancer cells overcome tumor TKI resistance. J Control Release. 2020;321:132-144 pubmed publisher
Schaefer T, Ramadoss A, Leu S, Tintignac L, Tostado C, Bink A, et al. Regulation of glioma cell invasion by 3q26 gene products PIK3CA, SOX2 and OPA1. Brain Pathol. 2019;29:336-350 pubmed publisher
Razavi P, Chang M, Xu G, Bandlamudi C, Ross D, VASAN N, et al. The Genomic Landscape of Endocrine-Resistant Advanced Breast Cancers. Cancer Cell. 2018;34:427-438.e6 pubmed publisher
Roof A, Jirawatnotai S, Trudeau T, Kuzyk C, Wierman M, Kiyokawa H, et al. The Balance of PI3K and ERK Signaling Is Dysregulated in Prolactinoma and Modulated by Dopamine. Endocrinology. 2018;159:2421-2434 pubmed publisher
Romano G, Chen P, Song P, McQuade J, Liang R, Liu M, et al. A Preexisting Rare PIK3CAE545K Subpopulation Confers Clinical Resistance to MEK plus CDK4/6 Inhibition in NRAS Melanoma and Is Dependent on S6K1 Signaling. Cancer Discov. 2018;8:556-567 pubmed publisher
Nisa L, Häfliger P, Poliaková M, Giger R, Francica P, Aebersold D, et al. PIK3CA hotspot mutations differentially impact responses to MET targeting in MET-driven and non-driven preclinical cancer models. Mol Cancer. 2017;16:93 pubmed publisher
Welsch M, Kaplan A, Chambers J, Stokes M, Bos P, Zask A, et al. Multivalent Small-Molecule Pan-RAS Inhibitors. Cell. 2017;168:878-889.e29 pubmed publisher
Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher
Stoy C, Sundaram A, Rios Garcia M, Wang X, Seibert O, Zota A, et al. Transcriptional co-factor Transducin beta-like (TBL) 1 acts as a checkpoint in pancreatic cancer malignancy. EMBO Mol Med. 2015;7:1048-62 pubmed publisher
Sathe A, Guerth F, Cronauer M, Heck M, Thalgott M, Gschwend J, et al. Mutant PIK3CA controls DUSP1-dependent ERK 1/2 activity to confer response to AKT target therapy. Br J Cancer. 2014;111:2103-13 pubmed publisher
Kohsaka S, Shukla N, Ameur N, Ito T, Ng C, Wang L, et al. A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations. Nat Genet. 2014;46:595-600 pubmed publisher
Zhao J, Liu Z, Wang L, Shin E, Loda M, Roberts T. The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005;102:18443-8 pubmed
product information
Catalog Number :
12525
Product Name :
pBabe puro HA PIK3CA E545K
article :
doi10.1073/pnas.0508988102
id548
pubmed_id16339315
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro
backbone_mutation
backbone_originAvailable at Addgene (plasmid #1764)
backbone_size5150
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Mutation generated using Strategene Quickchange site-directed mutagenesis kit. See article for primer information. Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val (I143V when compared to NP_006209.2). It could be a SNP. No functional changes have been observed so far in signaling assays.
origin :
37
pi :
alt_names
phosphoinositide 3-kinase
PI3K
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutationE545K. Tumor-derived allele.
namePIK3CA E545K
shRNA_sequence
size3277
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
613
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA