This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe puro HA PIK3CA H1047R
catalog :
12524
citations: 28
Reference
Hassenr xfc ck F, Farina Morillas M, Neumann L, Landini F, Blakemore S, Rabipour M, et al. Functional impact and molecular binding modes of drugs that target the PI3K isoform p110δ. Commun Biol. 2023;6:603 pubmed publisher
Wilson M, Harkins S, Reske J, Siwicki R, Adams M, Bae Jump V, et al. PIK3CA mutation in endometriotic epithelial cells promotes viperin-dependent inflammatory response to insulin. Reprod Biol Endocrinol. 2023;21:43 pubmed publisher
Keraite I, Alvarez García V, Leslie N. Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants. Methods Mol Biol. 2023;2621:41-56 pubmed publisher
Malik N, Kim Y, Yan H, Tseng Y, Du Bois W, Ayaz G, et al. Dysregulation of Mitochondrial Translation Caused by CBFB Deficiency Cooperates with Mutant PIK3CA and Is a Vulnerability in Breast Cancer. Cancer Res. 2023;83:1280-1298 pubmed publisher
Paul I, Bolzan D, Youssef A, Gagnon K, Hook H, Karemore G, et al. Parallelized multidimensional analytic framework applied to mammary epithelial cells uncovers regulatory principles in EMT. Nat Commun. 2023;14:688 pubmed publisher
Candido S, Salemi R, Piccinin S, Falzone L, Libra M. The PIK3CA H1047R Mutation Confers Resistance to BRAF and MEK Inhibitors in A375 Melanoma Cells through the Cross-Activation of MAPK and PI3K-Akt Pathways. Pharmaceutics. 2022;14: pubmed publisher
Young A, Bu W, Jiang W, Ku A, Kapali J, Dhamne S, et al. Targeting the Pro-survival Protein BCL-2 to Prevent Breast Cancer. Cancer Prev Res (Phila). 2022;15:3-10 pubmed publisher
Wilson M, Reske J, Holladay J, Neupane S, Ngo J, Cuthrell N, et al. ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer Hyperacetylation. Cell Rep. 2020;33:108366 pubmed publisher
Kutys M, Polacheck W, Welch M, Gagnon K, Koorman T, Kim S, et al. Uncovering mutation-specific morphogenic phenotypes and paracrine-mediated vessel dysfunction in a biomimetic vascularized mammary duct platform. Nat Commun. 2020;11:3377 pubmed publisher
Jayashankar V, Edinger A. Macropinocytosis confers resistance to therapies targeting cancer anabolism. Nat Commun. 2020;11:1121 pubmed publisher
Kinoh H, Shibasaki H, Liu X, Yamasoba T, Cabral H, Kataoka K. Nanomedicines blocking adaptive signals in cancer cells overcome tumor TKI resistance. J Control Release. 2020;321:132-144 pubmed publisher
Wilson M, Reske J, Holladay J, Wilber G, Rhodes M, Koeman J, et al. ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion. Nat Commun. 2019;10:3554 pubmed publisher
Lourenco C, Kalkat M, Houlahan K, De Melo J, Longo J, Done S, et al. Modelling the MYC-driven normal-to-tumour switch in breast cancer. Dis Model Mech. 2019;12: pubmed publisher
Schaefer T, Ramadoss A, Leu S, Tintignac L, Tostado C, Bink A, et al. Regulation of glioma cell invasion by 3q26 gene products PIK3CA, SOX2 and OPA1. Brain Pathol. 2019;29:336-350 pubmed publisher
Razavi P, Chang M, Xu G, Bandlamudi C, Ross D, VASAN N, et al. The Genomic Landscape of Endocrine-Resistant Advanced Breast Cancers. Cancer Cell. 2018;34:427-438.e6 pubmed publisher
Brandt L, Albers J, Hejhal T, Pfundstein S, Gonçalves A, Catalano A, et al. Mouse genetic background influences whether HrasG12V expression plus Cdkn2a knockdown causes angiosarcoma or undifferentiated pleomorphic sarcoma. Oncotarget. 2018;9:19753-19766 pubmed publisher
Alvarez García V, Bartos C, Keraite I, Trivedi U, Brennan P, Kersaudy Kerhoas M, et al. A simple and robust real-time qPCR method for the detection of PIK3CA mutations. Sci Rep. 2018;8:4290 pubmed publisher
Poli V, Fagnocchi L, Fasciani A, Cherubini A, Mazzoleni S, Ferrillo S, et al. MYC-driven epigenetic reprogramming favors the onset of tumorigenesis by inducing a stem cell-like state. Nat Commun. 2018;9:1024 pubmed publisher
Yoh K, Regunath K, Guzman A, Lee S, Pfister N, Akanni O, et al. Repression of p63 and induction of EMT by mutant Ras in mammary epithelial cells. Proc Natl Acad Sci U S A. 2016;113:E6107-E6116 pubmed
Henry W, Hendrickson D, Beca F, Glass B, Lindahl Allen M, He L, et al. LINC00520 is induced by Src, STAT3, and PI3K and plays a functional role in breast cancer. Oncotarget. 2016;7:81981-81994 pubmed publisher
Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher
Li L, Che L, Wang C, Blecha J, Li X, VanBrocklin H, et al. [(11)C]acetate PET Imaging is not Always Associated with Increased Lipogenesis in Hepatocellular Carcinoma in Mice. Mol Imaging Biol. 2016;18:360-7 pubmed publisher
Ricoult S, Yecies J, Ben Sahra I, Manning B. Oncogenic PI3K and K-Ras stimulate de novo lipid synthesis through mTORC1 and SREBP. Oncogene. 2016;35:1250-60 pubmed publisher
Li X, Tao J, Cigliano A, Sini M, Calderaro J, Azoulay D, et al. Co-activation of PIK3CA and Yap promotes development of hepatocellular and cholangiocellular tumors in mouse and human liver. Oncotarget. 2015;6:10102-15 pubmed
George S, Milea A, Sowamber R, Chehade R, Tone A, Shaw P. Loss of LKB1 and p53 synergizes to alter fallopian tube epithelial phenotype and high-grade serous tumorigenesis. Oncogene. 2016;35:59-68 pubmed publisher
Kohsaka S, Shukla N, Ameur N, Ito T, Ng C, Wang L, et al. A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations. Nat Genet. 2014;46:595-600 pubmed publisher
Magudia K, Lahoz A, Hall A. K-Ras and B-Raf oncogenes inhibit colon epithelial polarity establishment through up-regulation of c-myc. J Cell Biol. 2012;198:185-94 pubmed publisher
Zhao J, Liu Z, Wang L, Shin E, Loda M, Roberts T. The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005;102:18443-8 pubmed
product information
Catalog Number :
12524
Product Name :
pBabe puro HA PIK3CA H1047R
article :
doi10.1073/pnas.0508988102
id548
pubmed_id16339315
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro
backbone_mutation
backbone_originAvailable at Addgene (plasmid #1764)
backbone_size5150
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Mutation generated using Strategene Quickchange site-directed mutagenesis kit. See article for primer information. Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.
growth strain :
Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)
origin :
37
pi :
alt_names
phosphoinositide 3-kinase
PI3K
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutationH1047R. Tumor-derived allele.
namePIK3CA H1047R
shRNA_sequence
size3277
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
613
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA