This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe puro Myr HA PIK3CA
catalog :
12523
citations: 7
Reference
Mereness J, Bhattacharya S, Wang Q, Ren Y, Pryhuber G, Mariani T. Type VI collagen promotes lung epithelial cell spreading and wound-closure. PLoS ONE. 2018;13:e0209095 pubmed publisher
Högstrand K, Darmanin S, Forshell T, Grandien A. Transformation of mouse T cells requires MYC and AKT activity in conjunction with inhibition of intrinsic apoptosis. Oncotarget. 2018;9:21396-21410 pubmed publisher
Avanzato D, Pupo E, Ducano N, Isella C, Bertalot G, Luise C, et al. High USP6NL Levels in Breast Cancer Sustain Chronic AKT Phosphorylation and GLUT1 Stability Fueling Aerobic Glycolysis. Cancer Res. 2018;78:3432-3444 pubmed publisher
Di Franco S, Turdo A, Benfante A, Colorito M, Gaggianesi M, Apuzzo T, et al. ?Np63 drives metastasis in breast cancer cells via PI3K/CD44v6 axis. Oncotarget. 2016;7:54157-54173 pubmed publisher
Paul P, Volny N, Lee S, Qiao J, Chung D. Gli1 transcriptional activity is negatively regulated by AKT2 in neuroblastoma. Oncotarget. 2013;4:1149-57 pubmed
Hung S, Shih Y, Chen M, Lo S. Up-regulated cten by FGF2 contributes to FGF2-mediated cell migration. Mol Carcinog. 2014;53:787-92 pubmed publisher
Zhao J, Liu Z, Wang L, Shin E, Loda M, Roberts T. The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005;102:18443-8 pubmed
product information
Catalog Number :
12523
Product Name :
pBabe puro Myr HA PIK3CA
article :
doi10.1073/pnas.0508988102
id548
pubmed_id16339315
bacterial resistance :
Ampicillin
cloning :
backbonepBabe puro
backbone_mutation
backbone_originAvailable at Addgene (plasmid #1764)
backbone_size5150
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
To make an amino-terminal myristoylated allele of p110, a coding sequence for the myristoylation domain of c-Src was fused in-frame with a HA-tagged PIK3CA.
origin :
37
pi :
alt_names
phosphoinositide 3-kinase
PI3K
p110 alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5
promoter
sequencing_primer_3pBABE-3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
genePIK3CA
id5290
genbank_ids
mutationMyristoylation. Constitutively active allele; I143V
namePIK3CA Myr
shRNA_sequence
size3300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagMyr
locationN terminal on insert
tagHA
resistance markers :
613
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA