pBabe puro HA PIK3CA | Addgene 12522 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBabe puro HA PIK3CA

catalog :

12522

citations: 16

Reference
Hassenr xfc ck F, Farina Morillas M, Neumann L, Landini F, Blakemore S, Rabipour M, et al. Functional impact and molecular binding modes of drugs that target the PI3K isoform p110δ. Commun Biol. 2023;6:603 pubmed publisher
Prieto Garcia C, Hartmann O, Reissland M, Braun F, Bozkurt S, Pahor N, et al. USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K. Mol Oncol. 2022;: pubmed publisher
Candido S, Salemi R, Piccinin S, Falzone L, Libra M. The PIK3CA H1047R Mutation Confers Resistance to BRAF and MEK Inhibitors in A375 Melanoma Cells through the Cross-Activation of MAPK and PI3K-Akt Pathways. Pharmaceutics. 2022;14: pubmed publisher
Gjelaj E, Hamel P. Distinct epithelial-to-mesenchymal transitions induced by PIK3CAH1047R and PIK3CB. J Cell Sci. 2021;134: pubmed publisher
Ghezzi C, Wong A, Chen B, Ribalet B, Damoiseaux R, Clark P. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nat Commun. 2019;10:5444 pubmed publisher
Schaefer T, Ramadoss A, Leu S, Tintignac L, Tostado C, Bink A, et al. Regulation of glioma cell invasion by 3q26 gene products PIK3CA, SOX2 and OPA1. Brain Pathol. 2019;29:336-350 pubmed publisher
Razavi P, Chang M, Xu G, Bandlamudi C, Ross D, VASAN N, et al. The Genomic Landscape of Endocrine-Resistant Advanced Breast Cancers. Cancer Cell. 2018;34:427-438.e6 pubmed publisher
McNeill R, Stroobant E, Smithberger E, Canoutas D, Butler M, Shelton A, et al. PIK3CA missense mutations promote glioblastoma pathogenesis, but do not enhance targeted PI3K inhibition. PLoS ONE. 2018;13:e0200014 pubmed publisher
Lin R, Xia S, Shan C, Chen D, Liu Y, Gao X, et al. The Dietary Supplement Chondroitin-4-Sulfate Exhibits Oncogene-Specific Pro-tumor Effects on BRAF V600E Melanoma Cells. Mol Cell. 2018;69:923-937.e8 pubmed publisher
Romano G, Chen P, Song P, McQuade J, Liang R, Liu M, et al. A Preexisting Rare PIK3CAE545K Subpopulation Confers Clinical Resistance to MEK plus CDK4/6 Inhibition in NRAS Melanoma and Is Dependent on S6K1 Signaling. Cancer Discov. 2018;8:556-567 pubmed publisher
Henry W, Hendrickson D, Beca F, Glass B, Lindahl Allen M, He L, et al. LINC00520 is induced by Src, STAT3, and PI3K and plays a functional role in breast cancer. Oncotarget. 2016;7:81981-81994 pubmed publisher
Gámez B, Rodríguez Carballo E, Graupera M, Rosa J, Ventura F. Class I PI-3-Kinase Signaling Is Critical for Bone Formation Through Regulation of SMAD1 Activity in Osteoblasts. J Bone Miner Res. 2016;31:1617-30 pubmed publisher
Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, et al. Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumour formation in mice via AKT2/mTORC1 cascade. Liver Int. 2016;36:1176-86 pubmed publisher
Nakayama M, Inoue T, Naito M, Nakayama K, Ohara N. Attenuation of the phosphatidylinositol 3-kinase/Akt signaling pathway by Porphyromonas gingivalis gingipains RgpA, RgpB, and Kgp. J Biol Chem. 2015;290:5190-202 pubmed publisher
Leavenworth J, Verbinnen B, Yin J, Huang H, Cantor H. A p85Î±-osteopontin axis couples the receptor ICOS to sustained Bcl-6 expression by follicular helper and regulatory T cells. Nat Immunol. 2015;16:96-106 pubmed publisher
Zhao J, Liu Z, Wang L, Shin E, Loda M, Roberts T. The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Proc Natl Acad Sci U S A. 2005;102:18443-8 pubmed

product information

Catalog Number :

12522

Product Name :

pBabe puro HA PIK3CA

article :

doi	10.1073/pnas.0508988102
id	548
pubmed_id	16339315

bacterial resistance :

Ampicillin

cloning :

backbone

pBabe puro

backbone_mutation

backbone_origin

Available at Addgene (plasmid #1764)

backbone_size

5150

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

To construct a hemagglutinin (HA)-tagged allele of p110 alpha, a cDNA clone of human PIK3CA (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics [HIP]) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation. Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.

growth strain :

Retroviral expression of HA tagged phosphoinositide 3-kinase

origin :

pi :

alt_names

phosphoinositide 3-kinase

PI3K

p110 alpha

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBABE-3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CCM4, CLAPO, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha
gene	PIK3CA
id	5290

genbank_ids

mutation

name

PIK3CA

shRNA_sequence

size

3277

species

9606

Homo sapiens