This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFBOH-MHL.EED
catalog :
125174
citations: 1
Reference
Zhang Q, McKenzie N, Warneford Thomson R, Gail E, Flanigan S, Owen B, et al. RNA exploits an exposed regulatory site to inhibit the enzymatic activity of PRC2. Nat Struct Mol Biol. 2019;: pubmed publisher
product information
Catalog Number :
125174
Product Name :
pFBOH-MHL.EED
article :
doi10.1038/s41594-019-0197-y
id28197712
pubmed_id30833789
bacterial resistance :
Ampicillin
cloning :
backbonepFBOH-MHL baculovirus expression vector
backbone_mutationN-terminal His-MBP PreScission cleavage site
backbone_origina gift from the lab of Dr. Yufeng Tong, University of Toronto, Addgene #62304
backbone_size6767
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.
origin :
37
pi :
alt_names
Embryonic ectoderm development protein
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterPolyhedrin
sequencing_primer_33 sequencing primer pFBOH-rev 5 CTGATTATGATCCTCTAGTACTTCT 3
sequencing_primer_55 sequencing primer pFBOH-fwd 5 CCGGATTATTCATACCGTCCCACCA 3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCOGIS, HEED, WAIT1
geneEED
id8726
genbank_ids
8726
8726
mutation
namePolycomb protein EED
shRNA_sequence
size1326
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis-TEV
resistance markers :
4781
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA