This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCW57.1-DUXB-CA
catalog :
125027
citations: 1
Reference
Chew G, Campbell A, De Neef E, Sutliff N, Shadle S, Tapscott S, et al. DUX4 Suppresses MHC Class I to Promote Cancer Immune Evasion and Resistance to Checkpoint Blockade. Dev Cell. 2019;50:658-671.e7 pubmed publisher
product information
Catalog Number :
125027
Product Name :
pCW57.1-DUXB-CA
article :
doi10.1016/j.devcel.2019.06.011
id28197694
pubmed_id31327741
bacterial resistance :
Ampicillin
cloning :
backbonepCW57.1
backbone_mutation
backbone_origin
backbone_size9354
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
All-in-one lentivector with tet-inducible DUXB (codon sequences altered)
growth temp :
We used Stbl3 cells (recombination deficient)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NheI
promoterTRE
sequencing_primer_3agggctgccttggaaaag
sequencing_primer_5tcgcctggagaattggc
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneDUXB
id100033411
genbank_ids
NM_001351307
mutationcodon altered
nameDUXB (codon altered)
shRNA_sequence
size1038
species
9606
Homo sapiens
tags
resistance markers :
520
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA