This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p264 pSP72 hAR-LBD(LNCaP)
catalog :
12495
citations: 1
Reference
Kaczmarczyk S, Green J. Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Nucleic Acids Res. 2003;31:e86 pubmed
product information
Catalog Number :
12495
Product Name :
p264 pSP72 hAR-LBD(LNCaP)
article :
doi10.1093/nar/gng087
id892
pubmed_id12888538
bacterial resistance :
Ampicillin
cloning :
backbonepSP72
backbone_mutation
backbone_originPromega
backbone_size2500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Plasmid p258 was digested with BglI and BsgI and the 1.8 kb fragment was isolated. A second aliquot of p258 was digested with BglI and DraIII and the 1.3 kb fragment was isolated. Both of these fragments were ligated to a double-stranded oligonucleotide resulting in p264.
origin :
37
pi :
alt_names
androgen receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5XbaI
promoter
sequencing_primer_3T7
sequencing_primer_5SP6
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesAIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
geneAR
id367
genbank_ids
mutationThr 878 Ala in AR-LBD
nameAR LBD
shRNA_sequence
size903
species
9606
Homo sapiens
tags
resistance markers :
173
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA