p260 pCMV-CRE-M-AR(wt) | Addgene 12494 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p260 pCMV-CRE-M-AR(wt)

catalog :

12494

citations: 1

Reference
Kaczmarczyk S, Green J. Induction of cre recombinase activity using modified androgen receptor ligand binding domains: a sensitive assay for ligand-receptor interactions. Nucleic Acids Res. 2003;31:e86 pubmed

product information

Catalog Number :

12494

Product Name :

p260 pCMV-CRE-M-AR(wt)

article :

doi	10.1093/nar/gng087
id	892
pubmed_id	12888538

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-Cre-M (BglII)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Cre/Lox

growth notes :

To generate a fusion gene consisting of cre recombinase and the AR LBD, plasmid p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into pCMV-CRE-M(BglII) by digesting two separate aliquots of pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The ligation of these three fragments resulted in the generation of pCMV-CRE-M-AR(wt).

origin :

pi :

alt_names

androgen receptor

cloning

clone_method	Restriction Enzyme
cloning_site_3	See map
cloning_site_5	See map
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

mutation

CRE-M and AR ligand binding domain

name

CRE-M AR

shRNA_sequence

size

species

9606

Homo sapiens