This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTagGFP2-N SIN1 deltaPH
catalog :
124922
citations: 1
Reference
Hwang Y, Kim L, Song W, Edwards D, Cook R, Chen J. Disruption of scaffolding function of mLST8 selectively inhibits mTORC2 assembly and function and suppresses mTORC2-dependent tumor growth in vivo. Cancer Res. 2019;: pubmed publisher
product information
Catalog Number :
124922
Product Name :
pTagGFP2-N SIN1 deltaPH
article :
doi10.1158/0008-5472.CAN-18-3658
id28197675
pubmed_id31085701
bacterial resistance :
Kanamycin
cloning :
backbonepTagGFP2-N
backbone_mutation
backbone_originEvrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)
origin :
37
pi :
alt_names
SIN1
mSIN1
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5HindIII
promoter
sequencing_primer_3TagGFP-Rev, GCGCACGCTGAACTTGTGGC
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesJC310, MIP1, SIN1, SIN1b, SIN1g
geneMAPKAP1
id79109
genbank_ids
mutationInsert ORF was sirently mutated to be resistent to sgMAPKAP1_1.
nameMAPKAP1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagTagGFP2
resistance markers :
3625
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA