This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLentiCMV-Blast mLST8-Flag Mut1-2
catalog :
124918
citations: 1
Reference
Hwang Y, Kim L, Song W, Edwards D, Cook R, Chen J. Disruption of scaffolding function of mLST8 selectively inhibits mTORC2 assembly and function and suppresses mTORC2-dependent tumor growth in vivo. Cancer Res. 2019;: pubmed publisher
product information
Catalog Number :
124918
Product Name :
pLentiCMV-Blast mLST8-Flag Mut1-2
article :
doi10.1158/0008-5472.CAN-18-3658
id28197675
pubmed_id31085701
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV GFP Blast
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant
origin :
30
pi :
alt_names
GbetaL
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5XbaI
promoter
sequencing_primer_3WPRE-R
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGBL, GbetaL, LST8, POP3, WAT1
geneMLST8
id64223
genbank_ids
mutationInsert ORF was sirently mutated to be resistent to sgMLST8_1 and sgMLST8_4.
nameMLST8
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFlag
resistance markers :
3625
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA