This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAD64_pCMV6_Entry_hIrg1
catalog :
124879
citations: 1
Reference
Chen F, Lukat P, Iqbal A, Saile K, Kaever V, van den Heuvel J, et al. Crystal structure of cis-aconitate decarboxylase reveals the impact of naturally occurring human mutations on itaconate synthesis. Proc Natl Acad Sci U S A. 2019;116:20644-20654 pubmed publisher
product information
Catalog Number :
124879
Product Name :
pCAD64_pCMV6_Entry_hIrg1
article :
doi10.1073/pnas.1908770116
id28197659
pubmed_id31548418
bacterial resistance :
Kanamycin
cloning :
backbonepCMV6-Entry
backbone_mutation
backbone_originOrigene
backbone_size4831
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses hCAD His103Ala mutant in mammalian cells
origin :
37
pi :
alt_names
Irg1
CAD
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CAGGAAACAGCTATGACC
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCAD, IRG1
geneACOD1
id730249
genbank_ids
NM_001258406
mutationmutated His103 to Ala
namecis-aconitate decarboxylase
shRNA_sequence
size1443
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagmyc and FLAG tags
plasmid copy :
This plasmid was generated by mutagenesis of the plasmid CAT#: RC232825 purchased from Origene.
resistance markers :
58
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA