This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSR348
catalog :
124713
citations: 1
Reference
Schmidl S, Ekness F, Sofjan K, Daeffler K, Brink K, Landry B, et al. Rewiring bacterial two-component systems by modular DNA-binding domain swapping. Nat Chem Biol. 2019;15:690-698 pubmed publisher
product information
Catalog Number :
124713
Product Name :
pSR348
article :
doi10.1038/s41589-019-0286-6
id28197637
pubmed_id31110305
bacterial resistance :
Spectinomycin
cloning :
backbonepACYCDuet-1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Synthetic Biology
growth notes :
LacI is truncated and likely deactivated in this plasmid. This causes NarX, which is transcribed via the LacI repressible promoter Ptac, to be constitutively expressed.
growth strain :
Expression of narX under Ptac
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesb1222, ECK1216, frd1, frdR, narR
genenarX
id945788
genbank_ids
mutation
namenarX
shRNA_sequence
size1794
species
tags
alt_names
cloning
entrez_gene
aliasesb0345, ECK0342
genelacI
id945007
genbank_ids
mutationS193* - LacI is truncated and likely deactivated. Please see depositor comments below.
namelacI
shRNA_sequence
size1080
species
tags
resistance markers :
1868
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA