PD-L1-miSFIT-1x | Addgene 124685 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PD-L1-miSFIT-1x

catalog :

124685

citations: 1

Reference
Michaels Y, Barnkob M, Barbosa H, Baeumler T, Thompson M, Andre V, et al. Precise tuning of gene expression levels in mammalian cells. Nat Commun. 2019;10:818 pubmed publisher

product information

Catalog Number :

124685

Product Name :

PD-L1-miSFIT-1x

article :

doi	10.1038/s41467-019-08777-y
id	28197555
pubmed_id	30778069

bacterial resistance :

Ampicillin

cloning :

backbone

AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE

backbone_mutation

backbone_origin

Addgene: 85866

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Bi-directional expression vector driving PD-L1 under control of a miR-17 MRE with NGFR as an internal control

growth strain :

Tuning expression of PD-1

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter	minimal CMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
gene	CD274
id	29126

genbank_ids

mutation

name

PD-L1

shRNA_sequence

size

species

9606

Homo sapiens