pHO_pTEFmut7_CggR_R250A_ble | Addgene 124585 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHO_pTEFmut7_CggR_R250A_ble

catalog :

124585

citations: 3

Reference
Liu Y, Zhou Z, Wu S, Ni G, Zhang A, Tsimring L, et al. Enhanced cellular longevity arising from environmental fluctuations. bioRxiv. 2023;: pubmed publisher
Takhaveev V, xd6 zsezen S, Smith E, Zylstra A, Chaillet M, Chen H, et al. Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle. Nat Metab. 2023;5:294-313 pubmed publisher
Monteiro F, Hubmann G, Takhaveev V, Vedelaar S, Norder J, Hekelaar J, et al. Measuring glycolytic flux in single yeast cells with an orthogonal synthetic biosensor. Mol Syst Biol. 2019;15:e9071 pubmed publisher

product information

Catalog Number :

124585

Product Name :

pHO_pTEFmut7_CggR_R250A_ble

article :

doi	10.15252/msb.20199071
id	28197601
pubmed_id	31885198

bacterial resistance :

Ampicillin

cloning :

backbone

pHO - plasmid

backbone_mutation

Insertion of the ble selection marker (resistance against phleomycin)

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Synthetic Biology

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint

growth strain :

Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	TTT TGA TAC CGA GTT CGT TCT TTG TGG GGT
sequencing_primer_5	AAA ACT ATG GCT CAA GCA AGA AAC ACC CCA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BSU_33950, BSU33950
gene	cggR
id	938570

genbank_ids

mutation

changed arginine 250 to alanine

name

cggR_R250A

shRNA_sequence

size

species

100

Synthetic