This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTRE-tight2-Tmem98-FLAG
catalog :
124506
citations: 1
Reference
van der Wal T, Lambooij J, van Amerongen R. TMEM98 is a negative regulator of FRAT mediated Wnt/ß-catenin signalling. PLoS ONE. 2020;15:e0227435 pubmed publisher
product information
Catalog Number :
124506
Product Name :
pTRE-tight2-Tmem98-FLAG
article :
doi10.1371/journal.pone.0227435
id28197588
pubmed_id31961879
bacterial resistance :
Ampicillin
cloning :
backbonepTretight-2
backbone_mutationan SV40-puromycin-polyA resistance cassette was inserted into the backbone to allow selection in mammalian cells
backbone_originhttps://www.addgene.org/19407/
backbone_size4200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
growth strain :
Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promotertetO-CMV
sequencing_primer_3SV40 polyA
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases6530411B15Rik, AI463522
geneTmem98
id103743
genbank_ids
mutation
nameTmem98
shRNA_sequence
size770
species
10090
Mus musculus
tags
locationC terminal on insert
tagFlag
resistance markers :
4554
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA