This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tmem98-mTq2
catalog :
124504
citations: 1
Reference
van der Wal T, Lambooij J, van Amerongen R. TMEM98 is a negative regulator of FRAT mediated Wnt/ß-catenin signalling. PLoS ONE. 2020;15:e0227435 pubmed publisher
product information
Catalog Number :
124504
Product Name :
Tmem98-mTq2
article :
doi10.1371/journal.pone.0227435
id28197588
pubmed_id31961879
bacterial resistance :
Kanamycin
cloning :
backbonepAC-N2
backbone_mutation
backbone_origin
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector). To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted. The mTurquoise2 tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
growth strain :
Expresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SV40 polyA
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases6530411B15Rik, AI463522
geneTmem98
id103743
genbank_ids
mutationstop codon was removed to allow fusion with sGFP2 at the C-terminus
nameTmem98
shRNA_sequence
size770
species
10090
Mus musculus
tags
locationC terminal on insert
tagmTurquoise2
resistance markers :
4554
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA