This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGlomyc-Frat2
catalog :
124500
citations: 1
Reference
van der Wal T, Lambooij J, van Amerongen R. TMEM98 is a negative regulator of FRAT mediated Wnt/ß-catenin signalling. PLoS ONE. 2020;15:e0227435 pubmed publisher
product information
Catalog Number :
124500
Product Name :
pGlomyc-Frat2
article :
doi10.1371/journal.pone.0227435
id28197588
pubmed_id31961879
bacterial resistance :
Ampicillin
cloning :
backbonepGlomyc (based on pCDNA3.1)
backbone_mutationThe pGlomyc vector backbone contains 5 and 3 globin UTRs and the pCDNA1.1 BamHI-NsiI polylinker in the backbone of pCDNA3.1(+).
backbone_originOlivier Destree
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
References: https://www.ncbi.nlm.nih.gov/pubmed/?term=10534617 https://www.ncbi.nlm.nih.gov/pubmed/?term=15073180 https://www.ncbi.nlm.nih.gov/pubmed/?term=19802005 Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
growth strain :
Expresses myc-tagged mouse FRAT2 in mammalian cells (N-terminal tag)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3BGH polyA
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases
geneFrat2
id212398
genbank_ids
NM_177603.3
mutation
nameFrat2
shRNA_sequence
size696
species
10090
Mus musculus
tags
locationN terminal on insert
tagmyc tag
plasmid copy :
Construct was originally cloned in the lab of prof.dr. Anton Berns, Netherlands Cancer Institute.
resistance markers :
4554
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA