This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSuperRetro-EFP siRNA2
catalog :
12450
citations: 1
Reference
Zou W, Zhang D. The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. J Biol Chem. 2006;281:3989-94 pubmed
product information
Catalog Number :
12450
Product Name :
pSuperRetro-EFP siRNA2
article :
doi10.1074/jbc.M510787200
id885
pubmed_id16352599
bacterial resistance :
Ampicillin
cloning :
backbonepSuperRetroPuro
backbone_mutation
backbone_origin
backbone_size6349
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
RNAi
growth notes :
The targeted sequence for siRNA is EFP cDNA region 711-729 (ggtggagcagctacaacaa). The synthesized oligonucleotides is 5'-Gatccccggtggagcagctacaacaattcaag agattgttgtagctgctccaccttttta-3' and 5'-Agcttaaaaaggtggagcagctacaacaatct cttgaattgttgtagctgctccaccggg-3'.
origin :
37
pi :
alt_names
EFP
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5pBluescript KS
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEFP, RNF147, Z147, ZNF147
geneTRIM25
id7706
genbank_ids
mutation
namesiRNA against EFP
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
610
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA