This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFlagCMV2-EFP
catalog :
12449
citations: 5
Reference
Nasrullah U, Stanke K, Recknagel V, Bozkurt S, Wurzel P, Gauer S, et al. The E3 Ligase TRIM25 Impairs Apoptotic Cell Death in Colon Carcinoma Cells via Destabilization of Caspase-7 mRNA: A Possible Role of hnRNPH1. Cells. 2023;12: pubmed publisher
Airo A, Felix Lopez A, Mancinelli V, Evseev D, López Orozco J, Shire K, et al. Flavivirus Capsid Proteins Inhibit the Interferon Response. Viruses. 2022;14: pubmed publisher
Chang C, Liu H, Chang M, Chang S. Middle East Respiratory Syndrome Coronavirus Nucleocapsid Protein Suppresses Type I and Type III Interferon Induction by Targeting RIG-I Signaling. J Virol. 2020;94: pubmed publisher
Gupta S, Ylä Anttila P, Callegari S, Tsai M, Delecluse H, Masucci M. Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathog. 2018;14:e1006852 pubmed publisher
Zou W, Zhang D. The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. J Biol Chem. 2006;281:3989-94 pubmed
product information
Catalog Number :
12449
Product Name :
pFlagCMV2-EFP
article :
doi10.1074/jbc.M510787200
id885
pubmed_id16352599
bacterial resistance :
Ampicillin
cloning :
backbonepFlag-CMV2
backbone_mutation
backbone_origin
backbone_size4679
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human EFP cDNA subcloned into the blunt ended HindIII site of pFlag-CMV2.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoterCMV
sequencing_primer_3hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesEFP, RNF147, Z147, ZNF147
geneTRIM25
id7706
genbank_ids
mutation
nameEFP
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
610
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA