This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
catalog :
124299
citations: 1
Reference
Patil A, Manzano M, Gottwein E. Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma. Blood Adv. 2019;3:2105-2117 pubmed publisher
product information
Catalog Number :
124299
Product Name :
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
article :
doi10.1182/bloodadvances.2019031732
id28197556
pubmed_id31300418
bacterial resistance :
Ampicillin
cloning :
backbonepLCE
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3-
sequencing_primer_5-
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesE217K, UBC7, UBE2G
geneUBE2G1
id7326
genbank_ids
NM_003342
mutationseveral silent mutations to disrupt sgRNA targeting
nameUBE2G1
shRNA_sequence
size534
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
plasmid copy :
UBE2G1 cDNA was cloned from Addgene plasmid 31407
resistance markers :
4369
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA